β-HCG Elisa Kit

Description

β-HCG Elisa Kit

Product Overview: The β-HCG Elisa Kit is a diagnostic tool designed for the quantitative measurement of human chorionic gonadotropin (β-HCG) levels in serum or plasma samples. This enzyme-linked immunosorbent assay (ELISA) is widely used in clinical laboratories for the early detection of pregnancy, monitoring of trophoblastic diseases, and assessing the progression of various medical conditions related to β-HCG production. The kit is reliable, accurate, and easy to use, providing consistent results that are critical for patient diagnosis and care.

Key Features:

• High Sensitivity and Specificity: The β-HCG Elisa Kit offers high sensitivity, enabling the detection of low levels of β-HCG in early pregnancy or abnormal conditions.
• Quick Results: The test provides rapid results, typically within 90 minutes, ensuring timely medical intervention.
• Quantitative Measurement: It offers precise, quantifiable results, allowing for accurate monitoring of β-HCG concentrations.
• Easy-to-Use Protocol: The kit is designed for straightforward use, with minimal training required for healthcare professionals.
• Validated for Clinical Use: The β-HCG Elisa Kit is clinically validated and CE-marked, ensuring it meets rigorous quality and regulatory standards.
• Wide Range of Applications: Ideal for pregnancy testing, monitoring of gestational trophoblastic diseases, and evaluating other β-HCG-producing conditions.

Applications:

• Pregnancy Detection: Detection of β-HCG levels for early pregnancy confirmation.
• Trophoblastic Disease Monitoring: Used to diagnose and monitor conditions such as hydatidiform mole and choriocarcinoma.
• Fertility Assessment: Helps in evaluating fertility treatment progress by assessing β-HCG levels.
• Cancer Monitoring: Aids in the monitoring of certain cancers that produce β-HCG, including testicular and ovarian cancers.

Specifications:

• Type of Test: Enzyme-linked immunosorbent assay (ELISA)
• Sample Type: Serum or plasma
• Detection Range: 0 – 100,000 IU/L (varies based on specific kit version)
• Time to Results: Approximately 90 minutes
• Storage Conditions: Store at 2-8°C
• Shelf Life: 12-18 months (depending on lot number)

Components:

• 96-well microplate (coated with anti-β-HCG antibody)
• Calibrators and controls
• Conjugate reagent (anti-β-HCG-HRP)
• Substrate solution (TMB)
• Stop solution
• Wash buffer
• Sample diluent

Instructions for Use:

1. Prepare samples and reagents as per the provided instructions.
2. Add patient samples to the pre-coated microplate wells.
3. Incubate and wash according to the kit protocol.
4. Add the conjugate reagent and incubate again.
5. After further washing, add substrate solution and wait for color development.
6. Stop the reaction and measure the absorbance at 450 nm using a microplate reader.
7. Calculate the results based on the provided calibration curve.

Storage and Stability:

• Store the kit components at 2-8°C.
• Ensure that the reagents are not exposed to extreme temperatures.
• The reagents are stable until the expiration date printed on the kit packaging.

Safety Information:

• Follow standard laboratory safety procedures while handling biological samples and reagents.
• Use personal protective equipment (PPE) such as gloves, lab coat, and safety glasses during the test process.
• Dispose of all waste materials in accordance with local regulations.

Conclusion: The β-HCG Elisa Kit is a reliable and efficient tool for the detection and quantification of β-HCG in clinical settings. Its versatility makes it suitable for a wide range of diagnostic and monitoring applications, contributing to better patient care and clinical decision-making.