Human Growth Hormone Elisa Kit

Description

Human Growth Hormone ELISA Kit

Product Overview: The Human Growth Hormone (hGH) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of human growth hormone in serum, plasma, and cell culture supernatant. This enzyme-linked immunosorbent assay (ELISA) kit utilizes a solid-phase enzyme immunoassay technique to detect and measure hGH levels with precision and accuracy. It is widely used in research, clinical diagnostics, and pharmaceutical applications to monitor growth hormone levels and support the diagnosis of growth disorders, pituitary dysfunction, and other related conditions.

Key Features:

• Highly Sensitive: Accurate measurement of low concentrations of hGH in a variety of sample types.
• Wide Range: Suitable for a broad range of research and clinical applications.
• Fast and Reliable Results: Provides results in a short timeframe, ensuring efficient decision-making.
• Pre-coated Plates: Ready-to-use format with pre-coated antibodies, minimizing preparation time and effort.
• Quality Control: Includes positive and negative controls for validating assay performance.
• Customizable: Can be adapted for different sample types and volumes, offering flexibility in experimental design.

Applications:

• Clinical diagnostics for growth hormone deficiency or excess.
• Research on endocrine function and growth disorders.
• Investigation of pituitary diseases and hormone-related conditions.
• Pharmacological testing of potential growth hormone-related treatments.

Specifications:

• Format: 96-well microplate
• Detection Method: Colorimetric (OD at 450 nm)
• Assay Type: Sandwich ELISA
• Sample Type: Serum, plasma, and cell culture supernatants
• Sensitivity: 0.1 ng/mL
• Range: 0.1 ng/mL to 10 ng/mL
• Incubation Time: 90 minutes
• Storage Conditions: 2-8°C (reagents and kit components)

Instructions for Use:

1. Prepare reagents as indicated in the manual.
2. Add standards, controls, and samples to the microplate wells.
3. Incubate at room temperature and wash as per protocol.
4. Add enzyme-conjugate solution and incubate again.
5. Develop with substrate solution and read optical density at 450 nm.
6. Calculate the concentration of hGH in the samples using the standard curve.

Benefits:

• Non-radioactive and safe assay method.
• Easy-to-follow protocol, suitable for both novice and experienced users.
• Provides reproducible, reliable results for various sample types.
• Supports high-throughput testing and large-scale studies.

Storage and Stability:

• Store kit components at 2-8°C.
• Stable for 12 months when stored properly.