Alkaline Phosphatase

Description

Product Name: Alkaline Phosphatase (ALP)
CAS Number: 9001-78-9
Molecular Formula: Protein-based enzyme
Source: Bovine/Calf Intestinal Tissue (or other species, based on product specification)
Appearance: Lyophilized powder or liquid (depending on the product variant)
Storage Conditions: -20°C for long-term stability
Purity: ≥ 95% (SDS-PAGE analysis)
Activity Level: Customized units (e.g., 50 U/mg – 500 U/mg, depending on the application)

Product Description

Alkaline Phosphatase (ALP) is a hydrolytic enzyme widely utilized in biochemical and diagnostic research applications. It catalyzes the removal of phosphate groups from nucleotides, proteins, and alkaloids in alkaline conditions. This enzyme is instrumental in dephosphorylation processes, enabling its use in molecular biology, immunology, and clinical diagnostics.

Key Features:

• High Purity: Manufactured with stringent quality control for consistent performance.
• Optimal Activity: Designed for robust functionality across a range of experimental conditions.
• Multiple Forms Available: Available as lyophilized powder or stabilized liquid for flexibility in storage and handling.
• Source-Specific Options: Available from bovine intestinal or recombinant sources, tailored for specific applications.

Applications:

1. Molecular Biology:
   • Removal of 5’ phosphates in nucleic acids for cloning and labeling applications.
   • Vector dephosphorylation to prevent self-ligation in cloning experiments.
2. Clinical Diagnostics:
   • Biomarker for liver and bone-related conditions in serum assays.
   • Diagnostic kits for evaluating ALP activity in patient samples.
3. Immunoassays:
   • Enzyme-linked immunosorbent assays (ELISA) as a reporter enzyme.
   • Western blotting for signal detection in colorimetric or chemiluminescent assays.
4. Food and Pharmaceutical Industries:
   • Quality assurance in dairy products by detecting pasteurization efficacy.

Product Variants and Packaging:

• Standard Grades: Research and industrial-grade ALP tailored for specific applications.
• Custom Solutions: Activity and formulation customized to meet client-specific requirements.
• Packaging Sizes: Available in 10 mg, 50 mg, 100 mg, and bulk quantities for large-scale use.

Storage and Stability:

• Store at -20°C to maintain activity and stability.
• Lyophilized form provides enhanced shelf life and easy transport.
• Stable for 6-12 months under recommended conditions.

Safety and Handling:

• Use in a controlled laboratory setting.
• Follow standard safety protocols and wear appropriate personal protective equipment (PPE).
• Refer to the Safety Data Sheet (SDS) for detailed safety and disposal instructions.

Why Choose Our Alkaline Phosphatase?

• Rigorous quality assurance ensures reproducibility.
• Competitive pricing with flexible order sizes.
• Expert technical support for troubleshooting and application guidance.