Hantavirus-IgG Elisa Kit

Description

Product Information: Hantavirus-IgG ELISA Kit

Overview:
The Hantavirus-IgG ELISA Kit is a high-performance diagnostic tool designed for the detection of immunoglobulin G (IgG) antibodies specific to Hantavirus in human serum or plasma samples. This test enables the identification of past or recent exposure to Hantavirus, a potentially fatal virus that can cause hemorrhagic fever with renal syndrome (HFRS) or Hantavirus pulmonary syndrome (HPS). The ELISA (Enzyme-Linked Immunosorbent Assay) methodology used in this kit ensures high specificity, sensitivity, and reliability.

Key Features:

• Highly Sensitive & Specific: The kit offers excellent sensitivity and specificity for detecting Hantavirus IgG antibodies, reducing the risk of false positives and false negatives.
• Fast Results: Results can be obtained in just a few hours, making it an ideal tool for both routine and emergency testing.
• Convenient Format: Ready-to-use reagents and a simple step-by-step protocol ensure ease of use, even for labs with limited experience in ELISA testing.
• Reproducibility: The kit provides consistent and reliable results, even with repeated testing, ensuring accurate diagnostic outcomes.
• Validated for Human Samples: The kit has been validated for human serum and plasma samples, ensuring its applicability in clinical settings.

Applications:

• Clinical Diagnosis: Used in the diagnosis of Hantavirus infections, particularly in regions with endemic areas for Hantavirus diseases.
• Epidemiological Studies: Helps in conducting serological surveys and studies related to Hantavirus exposure and prevalence.
• Research Purposes: Ideal for research on Hantavirus immunology, disease transmission, and vaccine development.

Test Principle: The Hantavirus-IgG ELISA Kit utilizes an enzyme-labeled antigen to capture IgG antibodies specific to Hantavirus. The presence of these antibodies is then detected through a color change reaction, which is measured using a microplate reader. The intensity of the color correlates with the concentration of antibodies in the sample, providing quantitative results.

Kit Components:

• Coated Microplates (96 wells)
• Standard Solutions (Hantavirus IgG)
• Conjugate Reagent (Enzyme-labeled Antigen)
• Substrate Solution
• Wash Buffer
• Stop Solution
• Control Samples (Positive and Negative)

Storage and Stability:

• Store at 2-8°C (refrigeration) in a cool, dry place.
• The kit is stable for up to 12 months when stored properly.

Performance Characteristics:

• Sensitivity: 95%
• Specificity: 98%
• Detection Limit: 0.5 µg/mL of Hantavirus IgG antibodies

Instructions for Use:

1. Prepare the reagents as per the provided protocol.
2. Add serum or plasma samples to the microplate wells.
3. Incubate the plate with the conjugate reagent, wash thoroughly.
4. Add substrate solution and incubate until color develops.
5. Measure the absorbance at the specified wavelength using a microplate reader.

Safety Precautions:

• Follow appropriate biosafety guidelines when handling patient samples.
• Wear gloves, protective clothing, and safety glasses.
• Dispose of used materials according to local regulations.

Regulatory Compliance:

• CE Marked for in vitro diagnostic use.
• Complies with ISO 13485 standards for quality management in medical devices.

Packaging Options:

• Available in 96-well format.