HBeAg Elisa Kit

Description

Product Information: HBeAg ELISA Kit

Overview:
The HBeAg ELISA Kit is a highly sensitive and specific diagnostic tool designed to detect the presence of Hepatitis B e antigen (HBeAg) in human serum or plasma samples. This antigen is an important marker for the replication activity of the Hepatitis B virus (HBV), playing a crucial role in determining the infectivity and level of viral activity in individuals infected with HBV. The HBeAg ELISA Kit provides reliable results for clinicians to monitor the progress of HBV infection, assess viral replication, and guide treatment decisions.

Key Features:

• High Sensitivity and Specificity: Designed to provide accurate and reproducible results with minimal interference.
• Ease of Use: The kit includes all necessary reagents, controls, and standards required for performing the test, with a user-friendly protocol.
• Rapid Results: The ELISA assay is designed for efficient processing, allowing for timely diagnosis.
• Versatile: Suitable for use in clinical laboratories, research settings, and hospitals.

Applications:

• Hepatitis B Diagnosis: Detection of HBeAg in serum or plasma to identify active HBV infection.
• Monitoring Disease Progression: Helps assess viral replication and helps determine the level of infectivity in HBV-infected patients.
• Treatment Monitoring: Used to track the response to antiviral therapy in chronic Hepatitis B patients.

Principle of the Test:
This test is based on the enzyme-linked immunosorbent assay (ELISA) principle, utilizing a specific antibody to bind and detect the presence of HBeAg. The antigen-antibody reaction produces a color change, which is measured using a spectrophotometer. The intensity of the color correlates with the amount of HBeAg present in the sample.

Kit Components:

• Coated microplate (pre-coated with anti-HBeAg antibody)
• Standards and controls for calibration
• Conjugate solution (enzyme-labeled antibody)
• Substrate solution (for color development)
• Wash buffer and detection buffer

Storage and Handling:

• Store the kit at 2-8°C, away from direct sunlight and moisture.
• Ensure reagents are properly sealed and not exposed to extreme temperatures or contamination.

Test Procedure:

1. Add the samples and controls to the wells of the microplate.
2. Incubate the plate to allow binding between HBeAg and the immobilized antibody.
3. Wash the plate to remove unbound substances.
4. Add the enzyme-conjugate reagent and incubate.
5. Develop the color by adding substrate solution.
6. Measure absorbance at the appropriate wavelength to determine the presence and concentration of HBeAg.

Result Interpretation:
Results are based on the optical density (OD) readings from the spectrophotometer. A higher OD value indicates a higher concentration of HBeAg in the sample, indicating active viral replication.

Quality Assurance:
The HBeAg ELISA Kit is manufactured according to stringent quality control standards to ensure consistency and reliability in test results.

Important Notes:

• For in vitro diagnostic use only.
• Follow the instructions carefully to avoid errors and ensure accurate results.
• Dispose of all reagents and samples in accordance with local regulations.

Conclusion:
The HBeAg ELISA Kit offers a reliable and accurate solution for the detection and quantification of Hepatitis B e antigen. With its high sensitivity and simple protocol, it is an essential tool for diagnosing and monitoring Hepatitis B infections.